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KMID : 0381920070370030157
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Korean Journal of Microscopy
2007 Volume.37 No. 3 p.157 ~ p.166
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Effects of BCG on the Absorptive Cells in the Appendix of the Mouse Implanted with Ehrlich Carcinoma Cells
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Lee Woon-Woo
Park Kyung-Ho
Kim Myung-Soo
Park Dae-Kyun
Ko Jeong-Sik
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Abstract
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This experiment was performed to evaluate the ultrastructural responses of the absorptive cells in the appendix of the mouse, inoculated with Ehrlich carcinoma cells in the inguinal area, following administration of BCG (Bacillus Calmette-Guerin).
Healthy adult ICR mice weighing 25 gm each were divided into normal and experimental groups (experimental control group and BCG treated group). In the experimental groups, each mouse was inoculated with 1 ×107 Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day after inoculations, 0.5 mL of saline or BCG (0.5 mL/25 gm B.W.: 0.03×108~0.32×108 CFU) were injected subcutaneously to the animals every other day. The day following the last injection, each mouse was sacrificed. Pieces of the tissue were taken from the appendix, prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were stained with uranyl acetate and lead citrate.
In the normal control, experimental control and BCG treated mice, general morphology of the absorptive cells of appendix were similar. But myelin figures and intramitochondrial dense granules were more frequently observed in the absorptive cells of BCG treated mice than normal control ones. Above results show that BCG did show slight ultrastructural alterations in the absorptive cell of the appendix. These results that BCG may slightly suppress function of the absorptive cells of the appendix.
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KEYWORD
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Appendix, BCG (Bacillus Calmette-Guerin), Ehrlich carcinoma cell, Mouse, Ultrastructure
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